Insertion junction: LMJ.RY0402.097435_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097435
Chromosome:chromosome 12
Location:2367037
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g507500 (1 of 5) IPR001005//IPR009057//IPR017877 - SANT/Myb domain // Homeodomain-like // Myb-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCCCGTCAACCGTTTGTCAAACGATGT
Internal bar code:TAAGTACGAATCTGAACGGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:418
LEAP-Seq percent confirming:99.3512
LEAP-Seq n confirming:2297
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCATAAATGTGGGGCAG
Suggested primer 2:GATGCTGCTGTCATTGCTGT