| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097440 |
| Chromosome: | chromosome 12 |
| Location: | 7222787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558350 | (1 of 1) PF02609 - Exonuclease VII small subunit (Exonuc_VII_S) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTACGTATATCCATCTGCACACCTCATC |
| Internal bar code: | TGAGATGTCGTTATTTCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 98.5459 |
| LEAP-Seq n confirming: | 881 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACCGACCTACCTTGCAC |
| Suggested primer 2: | TGTTTGCTGTCTGCTTACGG |