| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097442 |
| Chromosome: | chromosome 6 |
| Location: | 7923465 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303400 | ADF3,FAP16 | (1 of 1) K18597 - echinoderm microtubule-associated protein-like 5 (EML5); necessary for axonemal severing | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGTGCCGTCGTTGAGCGCGATGGGCTT |
| Internal bar code: | TGTTTGCGGGTCTTGAAGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 99.6134 |
| LEAP-Seq n confirming: | 5669 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCTGCCTTACTTTGCC |
| Suggested primer 2: | AGCATGTCAGTCAGCCACAG |