Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.097448 |
Chromosome: | chromosome 5 |
Location: | 3011732 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g238400 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTAAACTCCCGCGAACTTATATTGTAAT |
Internal bar code: | CTCACGCTTTGGCCCAATGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 511 |
LEAP-Seq percent confirming: | 98.6646 |
LEAP-Seq n confirming: | 2512 |
LEAP-Seq n nonconfirming: | 34 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCTCTGCGACTGGTATGAC |
Suggested primer 2: | GCTTCAATCTTCACCCCAAA |