| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097459 |
| Chromosome: | chromosome 12 |
| Location: | 9724955 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557928 | (1 of 2) PF08592 - Domain of unknown function (DUF1772) (DUF1772) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATGCCGAGCGTGCGGACAAACTTATCG |
| Internal bar code: | GTTCGGGGTGGGCAGCCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 98.9011 |
| LEAP-Seq n confirming: | 3240 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCACTGAACTCTAGGC |
| Suggested primer 2: | GGCACCTTCGTTTCTGAGAG |