| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097462 |
| Chromosome: | chromosome 13 |
| Location: | 2876794 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583400 | OPR57 | OctotricoPeptide Repeat protein 57; (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCGTGCACCCCTGACCTGCCCTAGCG |
| Internal bar code: | CACGGTAACGCTTGAAATTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 98.028 |
| LEAP-Seq n confirming: | 2237 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGACTTCTGCCACGTCA |
| Suggested primer 2: | ATGGCAGAGATTTGAATGGG |