| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097474 |
| Chromosome: | chromosome 12 |
| Location: | 8762772 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545704 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTGACGTCGCATGGGTCCCCCAGAGAC |
| Internal bar code: | AGTTATTAAGCACACGCCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1117 |
| LEAP-Seq percent confirming: | 99.1159 |
| LEAP-Seq n confirming: | 1009 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATTAGCTCCGACAGGC |
| Suggested primer 2: | CCTACATGTCCGGCTTCATT |