Insertion junction: LMJ.RY0402.097488_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097488
Chromosome:chromosome 6
Location:3687173
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278140 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTGTTGCTCGCCTGTCCAGCCCGCATCC
Internal bar code:GACGCTCAGGCTTGTCGGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:99.2411
LEAP-Seq n confirming:2354
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTGACCTTCACCGTCCT
Suggested primer 2:AAGGACTTGAGGTGGTGTGG