| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097495 |
| Chromosome: | chromosome 12 |
| Location: | 1892193 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511200 | NUOS5 | NADH:ubiquinone oxidoreductase 10 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCTGTCCATGGCGCTGTGCCAGCCTGG |
| Internal bar code: | GACGGAATTGATGTTAAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.404 |
| LEAP-Seq n confirming: | 3669 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAAACTGCTTGAGGGCTT |
| Suggested primer 2: | CTGTGGAACAGTGGAAGGGT |