| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097500 |
| Chromosome: | chromosome 8 |
| Location: | 1187817 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363100 | (1 of 2) PTHR23301//PTHR23301:SF0 - CHITIN BINDING PERITROPHIN-A // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCGTGGGCAGCGCCGCCCGGACCCTG |
| Internal bar code: | CTTTCTATAAGTATTACGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.7521 |
| LEAP-Seq n confirming: | 1207 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTAAAGGCTGCACGGAA |
| Suggested primer 2: | GCCTCGATTGAGATTGTGGT |