| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097504 |
| Chromosome: | chromosome 12 |
| Location: | 3214199 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498800 | (1 of 1) PTHR23176:SF0 - RHO GTPASE-ACTIVATING PROTEIN 68F | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCATAACCCCACCTTGCGAACCTTCC |
| Internal bar code: | GGGGGGCACTGAGAGGAAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 98.4742 |
| LEAP-Seq n confirming: | 2388 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACTTCGACGGACATCCT |
| Suggested primer 2: | GTGTGGAGATGGACACATGC |