| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097514 |
| Chromosome: | chromosome 16 |
| Location: | 5006883 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCCTCCCCCTGCTCACATGCGGCCGCC |
| Internal bar code: | TTCTTCGAGATTTCGGCGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.7631 |
| LEAP-Seq n confirming: | 6316 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGATCCTCCCTACTCCA |
| Suggested primer 2: | TAGGGGCTGAAGAGGAGTCA |