| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097526 |
| Chromosome: | chromosome 9 |
| Location: | 1777646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396300 | PPX1,PPOX | (1 of 1) 1.3.3.4 - Protoporphyrinogen oxidase / Protoporphyrinogenase; Protoporphyrinogen oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTCGAAGTACCTTCAAAAGGGGGCTAT |
| Internal bar code: | AAGCCGTTGAAGTTGTGCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 99.602 |
| LEAP-Seq n confirming: | 2753 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAGTCTTGCGCTCCTTT |
| Suggested primer 2: | GAGCAGGAGGTGGTGCTAAG |