Insertion junction: LMJ.RY0402.097563_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.097563
Chromosome:chromosome 17
Location:3247140
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g722700 (1 of 2) PF04893 - Yip1 domain (Yip1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTTCTGGCGCTCCTCGCCGCGCCCCATC
Internal bar code:CACCCGTCGACGCGTTTCAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:9.7561
LEAP-Seq n confirming:204
LEAP-Seq n nonconfirming:1887
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTTTCAACGCATACCAG
Suggested primer 2:CGGACGTTGTAGAAGGGGTA