| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097564 |
| Chromosome: | chromosome 14 |
| Location: | 1677652 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619200 | (1 of 140) IPR002048 - EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGCCATGCTCGCGCCGTTCCCCTTGC |
| Internal bar code: | GAGGGCAGTCCTATCTCACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 97.7399 |
| LEAP-Seq n confirming: | 2465 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGGGTAGGCAGGGCATT |
| Suggested primer 2: | CATCCGTAGTGACGTTGTGG |