| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097586 |
| Chromosome: | chromosome 14 |
| Location: | 688446 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612500 | (1 of 1) K05954 - protein farnesyltransferase subunit beta (FNTB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGAGCAGGCATGTGGAGGAGTAGGGGT |
| Internal bar code: | TGCGGACAACTTTCACAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 90.1288 |
| LEAP-Seq n confirming: | 4547 |
| LEAP-Seq n nonconfirming: | 498 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTGTGTGCTCCTTGACA |
| Suggested primer 2: | GGAAACGTCTCAGGTGGTGT |