| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097596 |
| Chromosome: | chromosome 15 |
| Location: | 1295715 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641750 | (1 of 3) PF05495 - CHY zinc finger (zf-CHY) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTCACAGAGGCCAGAATGTAGCTCGCC |
| Internal bar code: | GGCACTTACCCGCCAGCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.7139 |
| LEAP-Seq n confirming: | 697 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCTAGCCACGAGGTTT |
| Suggested primer 2: | TGCAGAACTGTAGGTCGTCG |