| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097608 |
| Chromosome: | chromosome 3 |
| Location: | 8459697 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199983 | (1 of 1) PTHR11668//PTHR11668:SF257 - SERINE/THREONINE PROTEIN PHOSPHATASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGTTAGCTTACGGCAGCACCCTCACCGT |
| Internal bar code: | TGTCCGAAGTAAAGTGTGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 99.1272 |
| LEAP-Seq n confirming: | 7269 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACCTTCCAGAAATTTGA |
| Suggested primer 2: | TAGACGTCGGACTTTGCCTT |