| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097615 |
| Chromosome: | chromosome 14 |
| Location: | 4143569 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g634236 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTTTTGGGGGGTTTTACGCGTGCACC |
| Internal bar code: | TCGGCCCAACTTGTAGAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 99.7131 |
| LEAP-Seq n confirming: | 695 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGGGAACTCGTTTGACG |
| Suggested primer 2: | GCTATCTTAAGGCAGTGGCG |