| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097671 |
| Chromosome: | chromosome 10 |
| Location: | 2406381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435450 | CPL2 | (1 of 9) PTHR10071 - TRANSCRIPTION FACTOR GATA GATA BINDING FACTOR; transcription factor-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAATGCAGCAGCAGCAGCGGGGGACAG |
| Internal bar code: | CTGTAGGTCAGGGGCAGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.2565 |
| LEAP-Seq n confirming: | 1068 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGTCCCTGGACACTTT |
| Suggested primer 2: | GGTTTGACATGTTTGCGTTG |