| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097695 |
| Chromosome: | chromosome 6 |
| Location: | 3254505 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276650 | HFO2 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTCAAACATTGTGGTATGGGTTGGATTT |
| Internal bar code: | CGACTCCCCGTCAGCAACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.549 |
| LEAP-Seq n confirming: | 2428 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAACGGACTGACTGGGT |
| Suggested primer 2: | GGCCATTTTTGCTGAGTGAT |