| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097699 |
| Chromosome: | chromosome 3 |
| Location: | 6130351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191850 | SDR8 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.184//1.1.1.300 - Carbonyl reductase (NADPH) / Xenobiotic ketone reductase // NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCGTACCCACCAAGTTCAGCGCCGCGC |
| Internal bar code: | ATCGCAGCAAATGTTTGTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 98.2876 |
| LEAP-Seq n confirming: | 3329 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGCTTTACTCCCTTGAC |
| Suggested primer 2: | TGTACGCTGTCAGCAAGACC |