Insertion junction: LMJ.RY0402.097721_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097721
Chromosome:chromosome 6
Location:6245165
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g290900 (1 of 1) PF06294//PF11971 - CH-like domain in sperm protein (CH_2) // CAMSAP CH domain (CAMSAP_CH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCAGCACGTCTGTCCCTCAGGCAATAGA
Internal bar code:ATCCCCATTTCCCTTTACTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:652
LEAP-Seq percent confirming:99.3372
LEAP-Seq n confirming:2398
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTGCTGCTGGTAGGTGA
Suggested primer 2:GACCCACAAACAACTGGCTT