| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097730 |
| Chromosome: | chromosome 16 |
| Location: | 361909 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693819 | PWR12 | (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAAGAGTGGGTGTAGAGTTCATCGGGT |
| Internal bar code: | GTAATCGCAAATCTCCGGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 55.5141 |
| LEAP-Seq n confirming: | 1042 |
| LEAP-Seq n nonconfirming: | 835 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTATGCTGAGCCCCTTCAC |
| Suggested primer 2: | ACTGGTCCCGACAAAACAAG |