| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097733 |
| Chromosome: | chromosome 1 |
| Location: | 6031131 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042750 | ACH1 | (1 of 1) 4.2.1.3 - Aconitate hydratase / Citrate(isocitrate) hydro-lyase; Aconitate hydratase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCAAATGCCAACCCTTGGGTCGTTCGT |
| Internal bar code: | AGGATGCCATAGACCCGGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 22 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAAATAGACGTCGTGGT |
| Suggested primer 2: | ACCGGCAATTGACGAGTAAC |