| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097746 |
| Chromosome: | chromosome 16 |
| Location: | 583207 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692300 | (1 of 6) 3.6.3.43 - Peptide-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGCTCTTTACCTCGCAACGACAGTCGC |
| Internal bar code: | TAGCTTTATGGTGATCTAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 99.4416 |
| LEAP-Seq n confirming: | 2493 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTACCTGTCGTCGTGAGGG |
| Suggested primer 2: | TTTGCCCCAGTACCGTTAAG |