Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.097750 |
Chromosome: | chromosome 7 |
Location: | 70022 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g312580 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGAGGATGGGGGATTCGGAAGGGAGG |
Internal bar code: | GCTACCGCCCGGGCCCCCGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 255 |
LEAP-Seq percent confirming: | 86.2069 |
LEAP-Seq n confirming: | 325 |
LEAP-Seq n nonconfirming: | 52 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGACACACCACATCCAAC |
Suggested primer 2: | CTGGTGTTCTCCAGGTTGGT |