Insertion junction: LMJ.RY0402.097769_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.097769
Chromosome:chromosome 9
Location:2314628
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g392252 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCCCCCGCCTCCTCCCGCCCCTCTGCCG
Internal bar code:TTTTTCGACCCGTGTGTTAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:31
LEAP-Seq percent confirming:76.8817
LEAP-Seq n confirming:286
LEAP-Seq n nonconfirming:86
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCAACGGTAATAACGGC
Suggested primer 2:AGCGTGTGGACACTAATCCC