| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097822 |
| Chromosome: | chromosome 7 |
| Location: | 2888539 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332450 | (1 of 1) PTHR31528//PTHR31528:SF1 - FAMILY NOT NAMED // PYRIMIDINE PRECURSOR BIOSYNTHESIS ENZYME THI11-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCTTGCTGGTGAGCAGGCCGCTGGCG |
| Internal bar code: | CCCGGTGCTTGGGACTATATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 85.4701 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAACATCCCTGCTACCTC |
| Suggested primer 2: | GTTTTGCCTTGCTCACAACA |