| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097874 |
| Chromosome: | chromosome 7 |
| Location: | 491864 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315850 | (1 of 2) IPR000104//IPR000945//IPR020683 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGATATGACCGTGTGTGTGTTATGCC |
| Internal bar code: | CGTATCTGGCCTCCGTTTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATTGACCAAGTACCGCAA |
| Suggested primer 2: | CGTCTCTCCCTCACTTACGC |