| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097875 |
| Chromosome: | chromosome 8 |
| Location: | 4787269 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384285 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGAGTACATGGCCAAGGCCTGGCACTT |
| Internal bar code: | AGCGTCCAGGTTATTTCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 66.3236 |
| LEAP-Seq n confirming: | 1160 |
| LEAP-Seq n nonconfirming: | 589 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTGGTGACTGGTGAGT |
| Suggested primer 2: | TGTGTACGTGCAAGCTAGGC |