| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097918 |
| Chromosome: | chromosome 4 |
| Location: | 2024471 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218900 | (1 of 2) PTHR10887//PTHR10887:SF333//PTHR13779//PTHR13779:SF20 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED // HOLLIDAY JUNCTION DNA HELICASE RUVB-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTGTGCCTTTTTCAACCCTCACTGTAC |
| Internal bar code: | GCGGTTATGGCTGTCAGTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 99.7369 |
| LEAP-Seq n confirming: | 16300 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCATACAGGTTCGAGT |
| Suggested primer 2: | TTCGAGCTCAACTTCCGATT |