| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097919 |
| Chromosome: | chromosome 9 |
| Location: | 917246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401100 | (1 of 1) PTHR12560//PTHR12560:SF0 - LONGEVITY ASSURANCE FACTOR 1 LAG1 // LD18904P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCCTGCACGTGGCCAAGGTGGTGCAC |
| Internal bar code: | GTTTATTGTTCTTGCCGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 99.4159 |
| LEAP-Seq n confirming: | 8000 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATTACTGACACCCCGCTG |
| Suggested primer 2: | CTCTCTTCCCTTCCCTGTCC |