Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.097938 |
Chromosome: | chromosome 11 |
Location: | 1515467 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467747 | (1 of 2) PF10173 - Mitochondrial K+-H+ exchange-related (Mit_KHE1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAGAGCTGCGCAACAGGCACCTCAAGA |
Internal bar code: | AACACCCGGCGTTGACAGCGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 26 |
LEAP-Seq percent confirming: | 99.3525 |
LEAP-Seq n confirming: | 1074 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTGCAAGGAGCACCCTACT |
Suggested primer 2: | TGAAGGGGGAAAGAAGAGGT |