| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097946 |
| Chromosome: | chromosome 1 |
| Location: | 1026604 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005900 | (1 of 1) K10290 - F-box protein 3 (FBXO3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCGGGAGTCAGCCGTTCATGGACAAC |
| Internal bar code: | CGGTTACCCTCGTGGGGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 56.588 |
| LEAP-Seq n confirming: | 1151 |
| LEAP-Seq n nonconfirming: | 883 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATCACGGTGCGGAATAAG |
| Suggested primer 2: | ACATGGTAGGGAAGTCACGC |