| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097949 |
| Chromosome: | chromosome 1 |
| Location: | 675812 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003532 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGGGAGGGGTAGCCGTTCATGTGAAG |
| Internal bar code: | CAACCCTAGCACGAGTTGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 39.0533 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTGTGTGTGTGTGAGG |
| Suggested primer 2: | ATCCTCCTCCTCCTGGTCAT |