| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097988 |
| Chromosome: | chromosome 10 |
| Location: | 5801716 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461400 | (1 of 1) PTHR22957:SF212 - [PROTEIN ADP-RIBOSYLARGININE] HYDROLASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAATAGTGTCAAGGAGCGGTTCCTTGGA |
| Internal bar code: | TGTATGCTTGCAAGTCCTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 98.5765 |
| LEAP-Seq n confirming: | 277 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCCCGTCACCTCTAAA |
| Suggested primer 2: | GTCGGCAGTTGGTAGTCCAT |