| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098003 |
| Chromosome: | chromosome 12 |
| Location: | 1336297 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488752 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCTCAGTAGACATCACACTGGCAGCGC |
| Internal bar code: | ATGCTGTGCCACTCGCATTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 88.4174 |
| LEAP-Seq n confirming: | 771 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCGATCTCACCCACTTG |
| Suggested primer 2: | GCCGACAGTTCTCGAGGTTA |