| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098026 |
| Chromosome: | chromosome 6 |
| Location: | 409652 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252100 | (1 of 1) PTHR12389:SF0 - E3 UBIQUITIN-PROTEIN LIGASE LISTERIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGCCAGCCACCAGGGCCCCATGAGC |
| Internal bar code: | CCGGAAGACGGTCGTTGGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 66.25 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTACACACGGGTGATGC |
| Suggested primer 2: | CAGCAGAATGGGGAAGGATA |