| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098076 |
| Chromosome: | chromosome 3 |
| Location: | 4374554 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175300 | FAS14,FLA9 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; FAS1 domain containing protein; | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGGCCAGATGTGAGGATTGCCATAGT |
| Internal bar code: | TCCGGACGGTCTTTTTTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 99.6743 |
| LEAP-Seq n confirming: | 306 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTGTTTGGCTGCTTAC |
| Suggested primer 2: | CACACGGGCAAGACCTTATT |