Insertion junction: LMJ.RY0402.098131_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.098131
Chromosome:chromosome 16
Location:1359773
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g651900 CRI1,OPR129,OPR126,CRTISO OctotricoPeptide Repeat protein 129 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCAACCGCTACAGTCTGCAGATGCCATT
Internal bar code:ACTTCCGCGGAACCGTATGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:684
LEAP-Seq percent confirming:99.2832
LEAP-Seq n confirming:1108
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGTGTGTGTGGTTTCG
Suggested primer 2:CAGTCATTTGCTCGACTCCA