| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098149 |
| Chromosome: | chromosome 8 |
| Location: | 3848495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377700 | (1 of 7) PF02190 - ATP-dependent protease La (LON) substrate-binding domain (LON_substr_bdg) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCGCGTCTGGAGTGCCTGCCGGTCCTGC |
| Internal bar code: | TGAGTCATGCGAACCACAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 91.788 |
| LEAP-Seq n confirming: | 3655 |
| LEAP-Seq n nonconfirming: | 327 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTCTGCTCGTCTGGAGT |
| Suggested primer 2: | GCGCAATAGCAAGAAAGGAC |