| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.098176 |
| Chromosome: | chromosome 3 |
| Location: | 8545107 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201888 | (1 of 2) IPR027414 - Glycosyl hydrolase family, N-terminal domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCTGCACGGCGGCGGGAGGGCGAGTGT |
| Internal bar code: | ACTCAAACCGGGGCTGGAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 61.4396 |
| LEAP-Seq n confirming: | 239 |
| LEAP-Seq n nonconfirming: | 150 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGCTAGGGTCCATTGT |
| Suggested primer 2: | GCTGGACGAGTTGTAGAGGC |