Insertion junction: LMJ.RY0402.098185_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.098185
Chromosome:chromosome 5
Location:256295
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g233650 FXL9 (1 of 2) IPR000014//IPR011990 - PAS domain // Tetratricopeptide-like helical domain; FixL-like PAS domain protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGCATGAGGCTCGCTCATCCGCAGCCCC
Internal bar code:AAATCTAGTGGAAGCCTGTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:376
LEAP-Seq percent confirming:99.7428
LEAP-Seq n confirming:1551
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACCATGCTGTTTGCCCTAC
Suggested primer 2:GGGAGGAGAAGAGGGAAATG