Insertion junction: LMJ.RY0402.098189_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.098189
Chromosome:chromosome_3
Location:9158497
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g212305 FAP181 Flagellar Associated Coiled-Coil Protein antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):ACCCACCAGGTGTGCCGCGCGTGTCTCGGC
Internal bar code:TGCGGTTCCTCGGTCCGCGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:95
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTTGTTGCTGTGGGTGAT
Suggested primer 2:CGGTTTAGTTTGGGCTGGTA