Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.098190 |
Chromosome: | chromosome_10 |
Location: | 6072766 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g463370 | sense | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGGCCTCTTGTTAATTCCTCGCCGCGCCTC |
Internal bar code: | GATCGTTTCTCTGTGAGGGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 643 |
LEAP-Seq percent confirming: | 99.0758 |
LEAP-Seq n confirming: | 2680 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAACCCTGTATCTGTGCCT |
Suggested primer 2: | ATCGCCTCCACATACACACA |