Insertion junction: LMJ.RY0402.098192_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.098192
Chromosome:chromosome 3
Location:8305517
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g199100 (1 of 7) IPR001606 - ARID DNA-binding domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTCCAAGCACTGGCACCGGCACCACATT
Internal bar code:TATATGCAGCTGCGCTTGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:434
LEAP-Seq percent confirming:92.2222
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACGCACACACACACAC
Suggested primer 2:TTTCCGCTTCTGTGGCTACT