| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098212 |
| Chromosome: | chromosome 2 |
| Location: | 4026848 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097150 | AGG5 | (1 of 12) PF04749 - PLAC8 family (PLAC8); Aggregation 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACTGTTCTCCCCAATGCGGCCCTGCCT |
| Internal bar code: | GCCTGCGTGTGCCCCAGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 96.0084 |
| LEAP-Seq n confirming: | 457 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCCACTGTCTTTCTTGC |
| Suggested primer 2: | TGCTACTGTCATTCGTTCGC |