| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098259 |
| Chromosome: | chromosome 16 |
| Location: | 1921715 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656050 | (1 of 26) IPR000209 - Peptidase S8/S53 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAACGTACAGACATCAAACAGACTCCCG |
| Internal bar code: | GCGCTCGTGACATCTAGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 96.8182 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCACGTACCCCATGCTCT |
| Suggested primer 2: | GTATGGCGAGACCATCCAGT |