Insertion junction: LMJ.RY0402.098286_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.098286
Chromosome:chromosome 9
Location:75067
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g386250 SMM33 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTCGCTGGCCTGGATGCCGCTGGTTCTG
Internal bar code:CGGGATCAACGACGCATTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:97.3054
LEAP-Seq n confirming:325
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTACAAAACTTTGCGTGC
Suggested primer 2:AGGACGGTGTATTGTGGCTC